how prepare microscope slides for examination leaf cells

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How to prepare microscope slides for examination of leaf cells

This method describes how to prepare a peel of the leaf epidermis for microscopic observation of the epidermal cells, guard cells and leaf hairs if they are present. Plants vary in the shape of epidermal cells and size and number of stomata on each surface. If a good peel is obtained it is possible to estimate density of stomata. In leaves that have pigment in the vacuole, an epidermal peel can also be used to observe osmosis.